Small nucleolar (sno)RNAs are a large family of noncoding RNAs that often use antisense guide sequences to recognize target RNAs. The vast majority of targets for the ~2000 snoRNAs in humans remains unknown. Zhipeng Lu, Ph.D., and co-lead authors Minjie Zhang, Ph.D., and Kongpan Li, Ph.D., discuss improvements to genomic technologies that led to the identification and verification of a large network of snoRNA-tRNA interactions. These findings reveal a supply-and-demand economy where snoRNAs regulate tRNA usage to influence cell proliferation and development. Application of the improved PARIS2 and denatured RiboMeth-seq methods may allow dissection of other noncoding RNAs’ function in the human genome. Visit Zhipeng Lu’s Lab to learn more about work being conducted.
Read the full paper published in Proc Natl Acad Sci USA. 2023; 120 (41):e231216120..